CG100
Charge Variant Kit
A simple and robust option for separating protein charge variants in a pI range of 7.0–9.5.
A new approach to
analytical electrophoresis.
Personalised kits and method development tools to help you design your quality control and investigation processes, backed by world-class manufacturing and customer support.
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What is CE Genie?
Established
A new range of products for analytical customers from Helena Biosciences, with decades of CZE experience.
Capable
Enjoy peace of mind by relying on our global, battle-tested R&D, manufacturing and logistics capabilities.
Responsive
Our objective is to work with you to implement your process design and methods, with the possibility of personalised kit design.
CE Genie kits
Charge Variant Kit
REF CG100
The Capillary Zone Electrophoresis Charge Variant Analysis Kit delivers high-resolution, high-sensitivity separation of protein charge variants, enabling detailed characterisation of biotherapeutics with exceptional reproducibility and performance. Based on the proven method developed by He et al., the kit is specifically optimised for proteins with isoelectric points between pH 7.0–9.5, making it ideal for monoclonal antibody and therapeutic protein analysis.
The advanced background electrolyte formulation is designed to maximise charge differences between closely related variants, improving separation efficiency and analytical confidence. Dynamic coating components minimise protein adsorption and tightly control electroosmotic flow, supporting stable migration behaviour, enhanced resolution and highly reproducible results.
The kit also includes optimised conditioning reagents for consistent capillary performance and a dedicated charge variant control standard for system suitability testing and troubleshooting, helping streamline method setup and routine analysis.
Developed for demanding biopharmaceutical workflows, the kit provides a reliable, ready-to-use solution for sensitive charge variant profiling and protein quality assessment.
Kit contents
| Description | Volume |
|---|---|
| CZE Charge Variant Analysis Buffer | 140 mL |
| 0.1M HCl | 100 mL |
| 10mM Phosphoric acid | 100 mL |
| CZE Charge Variant Control | 2 mL |
CG200
IgG Heterogeneity and Purity Kit
Assess IgG purity in reduced and non-reduced forms using CE-CGE, for accurate size estimation and analyte identification.
IgG Heterogeneity and Purity Kit
REF CG200
Sodium Dodecyl Sulphate-Capillary Gel Electrophoresis (CE-SDS) delivers fast, high-resolution protein purity analysis by combining the trusted separation principles of SDS-PAGE with the automation, sensitivity and reproducibility of capillary electrophoresis. By separating proteins based on size with exceptional consistency and analytical performance, CE-SDS provides powerful insight into protein quality and structural integrity.
The kit supports both reduced and non-reduced analysis, enabling comprehensive characterisation of IgG and other biotherapeutics. Analysis of non-reduced proteins reveals intact protein purity, aggregates and structural variants, while reduced analysis provides detailed information on subunit composition, fragments, glycan occupancy and batch consistency. The kit includes an IgG control containing a defined amount of non-glycosylated heavy chain, enabling assessment of both assay resolution and quantitation performance before analysing unknown samples.
To support efficient sample preparation optimisation across a wide range of biotherapeutic samples, the kit also includes two sample preparation buffers at low and high pH. These complementary buffer conditions provide flexibility during method development, helping users identify optimal denaturation conditions for challenging proteins while improving sample stability, separation performance and reproducibility.
With automated capillary conditioning, fresh separation matrix for every run and reliable high-performance separations, CE-SDS is an essential tool for biopharmaceutical development, quality control and comparability studies.
Kit contents
| Description | Volume |
|---|---|
| SDS-MW separation gel buffer | 140 mL |
| SDS-MW sample preparation buffer | 50 mL |
| Low pH sample preparation buffer | 50 mL |
| 0.1M NaOH | 100 mL |
| 0.1M HCl | 100 mL |
| Internal standard | 0.4 mL |
| IgG Control | 1 mL |
CG300
CE-SDS Molecular Weight Kit
Profiles mAbs and biologics with a robust, high-resolution method suitable for development and QC.
CE-SDS Molecular Weight Kit
REF CG300
Capillary gel electrophoresis with sodium dodecyl sulphate delivers rapid, high-resolution protein analysis by combining the trusted separation principles of SDS-PAGE with the speed, automation and precision of capillary electrophoresis. Using advanced capillary technology and replaceable gel matrices, the kit enables highly reproducible separations with minimal sample consumption, making it ideal for modern biopharmaceutical and protein analysis workflows.
Proteins are uniformly coated with Sodium dodecyl sulphate (SDS), allowing separation based primarily on molecular size for accurate and reliable molecular weight determination. The high-performance sieving matrix provides excellent resolution across a defined separation range of 13-192kDa, enabling precise comparison against protein standards and confident identification of unknown proteins.
Beyond molecular weight analysis, the kit also supports protein quantification and purity assessment, providing a comprehensive solution for protein characterisation, quality control and biotherapeutic development.
To support efficient sample preparation optimisation across a wide range of protein samples, the kit also includes two sample preparation buffers at low and high pH. These complementary buffer conditions provide flexibility during method development, helping users identify optimal denaturation conditions for challenging proteins while improving sample stability, separation performance and reproducibility.
Kit contents
| Description | Volume |
|---|---|
| SDS-MW separation gel buffer | 140 mL |
| SDS-MW sample preparation buffer | 50 mL |
| Low pH sample preparation buffer | 50 mL |
| 0.1M NaOH | 100 mL |
| 0.1M HCl | 100 mL |
| Internal standard | 0.4 mL |
| Protein molecular weight standard | 1 mL |
CG400
Charge Variant Method Developer Kit
Simplify your development process with a range of BGEs, customisation options, and buffers and controls.
Charge Variant Method Developer Kit
REF CG400
The Capillary Zone Electrophoresis Charge Variant Method Development Kit is designed to simplify and optimise the separation of monoclonal antibody (mAb) and therapeutic protein charge variants, a critical challenge in modern biotherapeutic development.
This kit provides a powerful and flexible solution for developing molecule-specific charge variant separation methods through a carefully optimised range of background electrolytes (BGEs) with varying pH and ionic strengths. By enabling strategic pH optimisation relative to a protein’s isoelectric point (pI), the kit allows users to maximise charge-based separation selectivity and achieve enhanced resolution of closely related variants. This is particularly valuable for monoclonal antibodies and complex biotherapeutic proteins with pI values that fall outside the optimal separation window of conventional CZE methods.
The range of BGE pH and tuneable electrolyte compositions provided enables rapid screening and fine adjustment of separation conditions. Formulated with advanced buffering and dynamic coating components, the BGEs are designed to minimise protein adsorption, stabilise electroosmotic flow for improved migration consistency, and enhance separation reproducibility. Adjustable amine concentrations also allow rapid optimisation of polyamine levels for enhanced method development flexibility.
Ideal for biopharmaceutical characterisation, comparability studies and quality control, the kit enables reliable, high-resolution charge variant analysis and supports confident therapeutic protein development through tailored, pI-driven separation optimisation.
Kit contents
| Description | Volume |
|---|---|
| Buffer A: pH 5.2, low polyamine (2mM) | 140 mL |
| Buffer B: pH 5.2, high polyamine (10mM) | 140 mL |
| Buffer C: pH 5.7, mid polyamine (5mM) | 140 mL |
| Buffer D: pH 6.5, low polyamine (2mM) | 140 mL |
| Buffer E: pH 6.5, high polyamine (10mM) | 140 mL |
| Buffer F: pH 5.7, high polyamine (10mM), high buffering capacity | 140 mL |
| Buffer G: pH 6.6, high polyamine (10mM), high buffering capacity | 140 mL |
| 0.1M Hydrochloric acid | 100 mL |
| 10mM Phosphoric acid | 100 mL |
| CZE Charge Variant Control 1 | 2 mL |
| CZE Charge Variant Control 2 | 2 mL |
CG500
CZE Method Developer Kit
BGEs, capillary conditioning reagents, and controls for optimising CZE analysis of small molecules — in one developer kit.
CZE Method Developer Kit
REF CG500
The Capillary Electrophoresis Method Development Kit provides everything needed to accelerate and simplify CE method optimisation for small molecules, proteins and complex biomolecules. Designed to support both routine analysis and advanced method development, the kit combines carefully optimised background electrolytes, conditioning reagents and controls to help users achieve reliable, high-resolution separations with confidence.
With a versatile range of BGEs covering low pH, high pH, protein and MEKC applications, the kit allows rapid screening of separation conditions based on analyte charge, pKa or isoelectric point. Formulations provide strong buffering capacity, low UV absorbance and excellent reproducibility between analyses, while dynamic coating agents help control electroosmotic flow (EOF), reduce protein adsorption and improve separation stability.
The kit also supports separation of neutral compounds using micellar electrokinetic chromatography (MEKC), extending analytical capability beyond conventional CZE methods. Optimised conditioning reagents and guidance for capillary selection, temperature control and voltage application help users minimise method development time while maximising reproducibility and performance.
Whether developing high-resolution methods for biomolecules or rapid screening methods for small molecules, this comprehensive kit delivers the flexibility, control and consistency required for successful CE analysis.
Kit contents
| Description | Volume |
|---|---|
| 0.1M Hydrochloric acid | 100 mL |
| 0.1M Phosphoric acid | 100 mL |
| 0.1M NaOH | 100 mL |
| 50mM Borate, pH 9.3 | 125 mL |
| 50mM Borate, pH 9.3 + 100mM SDS | 125 mL |
| 100mM Phosphoric acid + 70mM Tris, pH 2.5 | 125 mL |
| 100mM Phosphoric acid + 70mM Triethanolamine, pH 2.5 + 0.1% Poloxamer 188. | 125 mL |
| Control for acidic buffers: 0.1mg/ml Bupivacaine.HCl | 2 mL |
| Control for basic buffers: 0.1mg/ml Benzoic acid | 2 mL |
Development service
Bespoke kit design
Our tailored customer service extends to customised or completely bespoke kit design. Contact us with your requirements to start the discussion.
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